SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Weckx,Lily Yin; Puccini,Rosana Fiorini; Machado,Antónia; Gonçalves,Maria Gisele; Tuboi,Suely; Barros,Eliana de; Devadiga,Raghavendra; Ortega-Barria,Eduardo; Colindres,Romulo.

ABSTRACT Meningococcal carriage is a prerequisite for invasive infection. This cross-sectional study assessed the pharyngeal carriage prevalence in healthy subjects aged 1-24 years in Embu das Artes city, São Paulo, Brazil. Pharyngeal swabs were examined for the presence of Neisseria meningitidis. The isolates were tested for different serogroups using agglutination and polymerase chain reaction. A logistic regression model assessed any independent association between Neisseria meningitidis carriage and various risk factors. A total of 87/967 subjects (9%, 95% Confidence Interval (CI): 7.3-11.0) tested positive for N. meningitidis: 6.2% (95% CI: 3.8-9.4) in 1-4 years, 8.5% (95% CI: 5.1-13.0) in 5-9 years, 12.5% (95% CI: 7.8-18.6) in 10-14 years, 12.6% (95%...

Carriage prevalence of Neisseria meningitidis in the Americas in the 21st century: a systematic review

Palavras-chave: Carriage; Meningococcal disease; Neisseria meningitidis; Pharyngeal; Serogroup.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600587

Santos-Neto,José Francisco; Ferreira,Viviane Matos; Feitosa,Caroline Alves; Martinez-Silveira,Martha Silvia; Campos,Leila Carvalho.

Abstract Neisseria meningitidis is a bacterium that colonizes the human nasopharynx and is transmitted by respiratory droplets from asymptomatic or symptomatic carriers. Occasionally, the pathogen invades the mucosa and enters the bloodstream, causing invasive meningococcal disease, a life-threatening infection. While meningococcal colonization is the first step in the development of invasive disease, the risk factors that predict progression from asymptomatic to symptomatic status are not well-known. The present report aimed to describe the prevalence of N. meningitidis carriers throughout the Americas, emphasizing the risk factors associated with carrier status, as well as the most prevalent serogroups in each studied population. We conducted a...

Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies

Palavras-chave: Neisseria meningitidis; Carrier; America; Epidemiology; Vaccine.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000400254

Belo,Elza FT; Farhat,Calil K; Gaspari,Elizabeth N De.

Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Brazilian epidemic). The dot-ELISA is based on the same principles as the standard ELISA and is useful for detection of anti-N. meningitidis B antibodies in serum of patients with meningococcal infections. For the assay, outer membrane complexes (OMCs) were absorbed by nitrocellulose membrane and blocked with a 5% skim milk solution. Serum samples were drawn upon...

Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples

Palavras-chave: Neisseria meningitidis; Dot-ELISA; ELISA; Antibodies; Outer membrane complex.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100008

de Filippis,Ivano; Andrade,Claudia Ferreira de; Caldeira,Nathalia; Azevedo,Aline Carvalho de; Almeida,Antonio Eugenio de.

Abstract Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans. Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers...

Deficiency of the eighth component of complement associated with recurrent meningococcal meningitis: case report and literature review

Palavras-chave: Diagnostic; Neisseria meningitidis; Streptococcus pneumoniae; Haemophilus influenzae.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400335

Rosa,Daniela Dornelles; Pasqualotto,Alessandro C.; Quadros,Maurício de; Prezzi,Sérgio Henrique.

The authors report a case of deficiency of the eighth component of complement in a young adult with a history of three episodes of meningitis; one of them proved to be meningococcal. The literature was reviewed and meningitis due to Neisseria meningitidis strains causing disease in complement-deficient and complement-sufficient patients was demonstrated. Meningococcal disease may be the first manifestation of complement deficiency; screening for complement function must be considered for those with invasive meningococcal disease, with posterior evaluation of the components of the terminal pathway of complement.

Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies

Palavras-chave: Meningococcal disease; Neisseria meningitidis; Complement deficiency.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400010

De Gaspari,Elizabeth N.; Zollinger,Wendell D..

Determining the profile of antigen expression among meningococci is important for epidemiologic surveillance and vaccine development. To this end, two new mouse monoclonal antibodies (MAbs) have been derived against Neisseria meningitidis proteins (class 5). The MAbs were reactive against outer membrane antigens and were bactericidal. Selected anti-class 5 MAbs [(5.1)-3E6-2; (5.3)-3BH4-C7; (5.4)-1BG11-C7; (5.5)-3DH-F5G9 also 5F1F4-T3(5.c)], and the two new monoclonal antibodies C14F10Br2 (5.8) and 7F11B5Br3 (5.9), were then tested against different meningococcal strains, (63 strains of serogroup A, 60 strains of serogroup C (from 1972 to 1974); and 136 strains of serogroup B (from 1992) meningococci). Our results demonstrated that the expression of class 5...

Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever

Palavras-chave: Neisseria meningitidis; Monoclonal antibodies; Outer membrane protein; Flow cytometry.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300007

BJM

Cury,Gisele Cristiane Gentile; Pereira,Rafaella Fabiana Carneiro; Hollanda,Luciana Maria de; Lancellotti,Marcelo.

The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the...

Microbiological epidemiological history of meningococcal disease in Rio de Janeiro, Brazil

Palavras-chave: Brazilian purpuric fever; Haemophilus influenzae biogroup aegyptius; Genetic transfer; Neisseria meningitidis; Haemophilus influenzae.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040

Barroso,David Eduardo; Carvalho,DM; Casagrande,ST; Rebelo,MC; Soares,V; Zahner,V; Solari,CA; Nogueira,SA.

The main objectives of the present study were to investigate the clinical and laboratory features of meningococcal disease in the city of Rio de Janeiro, Brazil, during the overlap of 2 epidemics in the 1990s. We conducted a study of a series of cases of meningococcal disease admitted in a Meningitis Reference Hospital. All clinical isolates available were analyzed by means of microbiological epidemiological markers. In 1990, Neisseria meningitidis serogroup B:4,7:P1.19,15, 1.7,1 sulfadiazine-resistant of the ET-5 complex emerged causing epidemic disease. Despite mass vaccination campaign (VaMengoc B+C®), the ET-5 clone remained hyperendemic after the epidemic peaked. In 1993 to 1995, an epidemic of serogroup C belonged to the cluster A4 overlapped, with a...

Neisseria meningitidis: a neglected cause of infectious haemorrhagic fever in the amazon rainforest

Palavras-chave: Neisseria meningitidis; Meningococcal disease; Epidemic; Meningitis; Sepsis.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300008

Barroso,David E.; Silva,Luciete A..

Neisseria meningitidis has not been seen as a significant cause of infectious haemorrhagic fever in the Amazon inlands; most reported cases are from the city of Manaus, the capital of the State of Amazonas. This picture is sustained by the lack of reliable microbiology laboratories, the perception of the health care workers, and the difficult to reach medical assistance; thus the number of confirmed cases is even lower with no reference of the strains phenotype. We report here the investigation of a case of suspected meningococcemia and his close contacts in a rural community in the Coari Lake, up the Amazon River.

Neisseria meningitidis presenting as acute abdomen and recurrent reactive pericarditis

Palavras-chave: Neisseria meningitidis; Meningococcal disease; Haemorrhagic fever; Amazon forest.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600014

Akinosoglou,Karolina; Alexopoulos,Angelos; Koutsogiannis,Nikolaos; Gogos,Charalampos; Lekkou,Aleksandra.

ABSTRACT Meningococcal meningitis is a well established potential fatal infection characterized by fever, headache, petechial rash, and vomiting in the majority of cases. However, protean manifestations including abdominal pain, sore throat, diarrhea and cough, even though rare, should not be overlooked. Similarly, although disseminated infection could potentially involve various organ-targets, secondary immune related complications including joints or pericardium should be dealt with caution, since they remain unresponsive to appropriate antibiotic regimens. We hereby report the rare case of an otherwise healthy adult female, presenting with acute abdominal pain masking Neisseria meningitidis serotype B meningitis, later complicated with recurrent...

Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media

Palavras-chave: Reactive pericarditis; Neisseria meningitidis; Meningococcal meningitis.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600641

BJM

Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista; Raw,Isaías.

Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and...

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Palavras-chave: Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007

Belo,Elza F.T.; Coutinho,Ligia M.C.C.; Ferraz,Aline S.; De Gaspari,Elizabeth N..

A new monoclonal antibody (5F81A4P1.9), which is specific for subtype 9 antigen of meningococci, was studied. The antibodies were raised against a previously non-typable (NT) serogroup B strain from Brazilian patients and were found to react with the subtype antigen of prototype reference strains for subtype 9 (M982), as well as with those of homologous strains. The subtype 9 epitope was found in 6.8% of serogroup B strains among 602 strains of Neisseria meningitidis case isolates, including representative isolates from Brazilian states. Subtype P1.9 was predominantly related to serogroup B in Brazil among the isolates collected during the N. meningitidis epidemic in 1992. No significant differences were observed in the occurrence of subtype P1.9 among...

Production of outer membrane vesicles (OMV) in batch cultivation of Neisseria meningitidis serogroup B

Palavras-chave: Neisseria meningitidis; Monoclonal antibodies; Brazilian meningococcus strains; Serotyping; Subtyping.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600004

BJM

Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado.

Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass...

Seroprevalence of bactericidal antibodies against serogroup B and C Meningococci in a University Hospital

Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016

BJMBR

Gioia,C.A.C.; Lemos,A.P.S.; Gorla,M.C.O.; Mendoza-Sassi,R.; Figueredo,B.S.; Ballester,T.; Von Groll,A.; Wedig,B.; Ethur,N.V.; Bragança,L.; Silva,P.E.A.; Milagres,L.G..

Meningococcus serogroup B (MenB), clonal complex 32 (cc 32), was the Brazilian epidemic strain of meningococcal disease (MD) in the 1990’s. Currently, meningococcus serogroup C (MenC), cc 103, is responsible for most of the cases of the disease in Brazil. The aim of this study was to investigate the seroprevalence of bactericidal antibody (SBA) against representative epidemic strains of MenC, (N753/00 strain, C:23:P1.22,14-6, cc103) and MenB, (Cu385/83 strain, B:4,7:P1.15,19, cc32) in students and employees of a university hospital in the State of Rio Grande do Sul (RS, Brazil). A second MenC strain (N79/96, C:2b:P1.5-2,10, cc 8) was used as a prototype strain of Rio de Janeiro’s outbreak that occurred in the 1990’s. Our previous study showed a 9% rate of...

Subclinical infection of the genital tract with Neisseria meningitidis

Palavras-chave: Neisseria meningitidis; Bactericidal antibodies; Meningococcal carrier.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500607

Lourenço,Maria Cristina S.; Reis,Roberto S.; Andrade,Angela C. V.; Tuyama,Mari; Barroso,David E.

We report the isolation of Neisseria meningitidis, characterized as B:NT:P1.7, from a female patient's genital tract in an outpatient clinic for HIV care. The gynecology clinic, as part of the follow up, collects specimens from all patients with HIV infection for routine exams and for early laboratory detection of sexually transmitted diseases . A Gram-negative diplococcus was isolated from the cervix of a heterosexual patient with AIDS. Based on this and other reported cases, urogenital infection with N. meningitidis can no longer be considered uncommon. The rising incidence of N. meningitidis isolated from this and similar sites has significant medical and diagnostic implications.